PubMed banner
PubMed Nucleotide Protein Genome Structure PopSet Taxonomy OMIM
 Search for
  Limits Preview/Index History Clipboard    
About Entrez
spacer gif
back to About Entrez
back to About Entrez

Entrez PubMed
Help | FAQ

PubMed Services
Journal Browser
MeSH Browser
Single Citation Matcher
Batch Citation Matcher
Clinical Queries

Related Resources
Order Documents
Grateful Med
Consumer Health
Clinical Alerts

Privacy Policy


1: IEEE Trans Biomed Eng 1998 Apr;45(4):422-8 Related Articles, Books, LinkOut

Filter matrix estimation in automated DNA sequencing.

Huang W, Fuhrmann DR, Politte DG, Thomas LJ Jr, States DJ

Institute for Biomedical Computing, Washington University, St. Louis, MO 63110, USA.

In four-color fluourescence-based automated DNA sequencing, a 4 x 4 filter matrix parameterizes the relationship between the dye-intensity signals of interest and the data collected by an optical imaging system. The filter matrix is important because the estimated DNA sequence is based on the dye intensities that can only be recovered via inversion of the matrix. In this paper, we present a calibration method for the estimation of the columns of this matrix, using data generated through a special experiment in which DNA samples are labeled with only one fluorescent dye at a time. Simulations and applications of the method to real data are provided, with promising results.

PMID: 9556959, UI: 98217707